eclipse 800 epifluorescence-equipped light microscope Search Results


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Vector Laboratories vectashield antifade medium
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CellASIC Corporation microfluidics device
Microfluidics Device, supplied by CellASIC Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hamamatsu orca 100 ccd camera
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Olympus ix83 inverted microscope system
Vav1 and 2 are expressed in MDMs and not affected by DENV infection. MDMs were left uninfected (UI) or DENV-infected, and at 48 hpi, cells were formalin fixed and then stained with (A) mouse-anti-NS1 (green) and rabbit-anti-Vav (total) (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively; (B) mouse-anti-NS1 (green) and rabbit-anti-Vav2 (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively. Nuclei were stained using Hoechst 33342 (blue). Images were captured (Olympus, <t>IX83),</t> and representative images are shown. (C) Images were assessed for protein colocalization by confocal microscopy (Zeiss LSM 880 with Airyscan), 63× objective lens at 2× zoom and Z-stack analysis. Two serial 0.25 µM images are shown for NS1/Vav2 staining from two different images for DENV-infected cells.
Ix83 Inverted Microscope System, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nikon dxm1200 ccd high resolution color camera
Vav1 and 2 are expressed in MDMs and not affected by DENV infection. MDMs were left uninfected (UI) or DENV-infected, and at 48 hpi, cells were formalin fixed and then stained with (A) mouse-anti-NS1 (green) and rabbit-anti-Vav (total) (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively; (B) mouse-anti-NS1 (green) and rabbit-anti-Vav2 (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively. Nuclei were stained using Hoechst 33342 (blue). Images were captured (Olympus, <t>IX83),</t> and representative images are shown. (C) Images were assessed for protein colocalization by confocal microscopy (Zeiss LSM 880 with Airyscan), 63× objective lens at 2× zoom and Z-stack analysis. Two serial 0.25 µM images are shown for NS1/Vav2 staining from two different images for DENV-infected cells.
Dxm1200 Ccd High Resolution Color Camera, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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KEYENCE keyence bz-800
Vav1 and 2 are expressed in MDMs and not affected by DENV infection. MDMs were left uninfected (UI) or DENV-infected, and at 48 hpi, cells were formalin fixed and then stained with (A) mouse-anti-NS1 (green) and rabbit-anti-Vav (total) (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively; (B) mouse-anti-NS1 (green) and rabbit-anti-Vav2 (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively. Nuclei were stained using Hoechst 33342 (blue). Images were captured (Olympus, <t>IX83),</t> and representative images are shown. (C) Images were assessed for protein colocalization by confocal microscopy (Zeiss LSM 880 with Airyscan), 63× objective lens at 2× zoom and Z-stack analysis. Two serial 0.25 µM images are shown for NS1/Vav2 staining from two different images for DENV-infected cells.
Keyence Bz 800, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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LI-COR odyssey
Vav1 and 2 are expressed in MDMs and not affected by DENV infection. MDMs were left uninfected (UI) or DENV-infected, and at 48 hpi, cells were formalin fixed and then stained with (A) mouse-anti-NS1 (green) and rabbit-anti-Vav (total) (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively; (B) mouse-anti-NS1 (green) and rabbit-anti-Vav2 (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively. Nuclei were stained using Hoechst 33342 (blue). Images were captured (Olympus, <t>IX83),</t> and representative images are shown. (C) Images were assessed for protein colocalization by confocal microscopy (Zeiss LSM 880 with Airyscan), 63× objective lens at 2× zoom and Z-stack analysis. Two serial 0.25 µM images are shown for NS1/Vav2 staining from two different images for DENV-infected cells.
Odyssey, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apple inc macintosh quadra 800 computer
Vav1 and 2 are expressed in MDMs and not affected by DENV infection. MDMs were left uninfected (UI) or DENV-infected, and at 48 hpi, cells were formalin fixed and then stained with (A) mouse-anti-NS1 (green) and rabbit-anti-Vav (total) (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively; (B) mouse-anti-NS1 (green) and rabbit-anti-Vav2 (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively. Nuclei were stained using Hoechst 33342 (blue). Images were captured (Olympus, <t>IX83),</t> and representative images are shown. (C) Images were assessed for protein colocalization by confocal microscopy (Zeiss LSM 880 with Airyscan), 63× objective lens at 2× zoom and Z-stack analysis. Two serial 0.25 µM images are shown for NS1/Vav2 staining from two different images for DENV-infected cells.
Macintosh Quadra 800 Computer, supplied by apple inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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BUCHI AG rotavapor buchi
Vav1 and 2 are expressed in MDMs and not affected by DENV infection. MDMs were left uninfected (UI) or DENV-infected, and at 48 hpi, cells were formalin fixed and then stained with (A) mouse-anti-NS1 (green) and rabbit-anti-Vav (total) (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively; (B) mouse-anti-NS1 (green) and rabbit-anti-Vav2 (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively. Nuclei were stained using Hoechst 33342 (blue). Images were captured (Olympus, <t>IX83),</t> and representative images are shown. (C) Images were assessed for protein colocalization by confocal microscopy (Zeiss LSM 880 with Airyscan), 63× objective lens at 2× zoom and Z-stack analysis. Two serial 0.25 µM images are shown for NS1/Vav2 staining from two different images for DENV-infected cells.
Rotavapor Buchi, supplied by BUCHI AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hamamatsu digital camera c11440 orca-flash 4.0
Vav1 and 2 are expressed in MDMs and not affected by DENV infection. MDMs were left uninfected (UI) or DENV-infected, and at 48 hpi, cells were formalin fixed and then stained with (A) mouse-anti-NS1 (green) and rabbit-anti-Vav (total) (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively; (B) mouse-anti-NS1 (green) and rabbit-anti-Vav2 (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively. Nuclei were stained using Hoechst 33342 (blue). Images were captured (Olympus, <t>IX83),</t> and representative images are shown. (C) Images were assessed for protein colocalization by confocal microscopy (Zeiss LSM 880 with Airyscan), 63× objective lens at 2× zoom and Z-stack analysis. Two serial 0.25 µM images are shown for NS1/Vav2 staining from two different images for DENV-infected cells.
Digital Camera C11440 Orca Flash 4.0, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Vav1 and 2 are expressed in MDMs and not affected by DENV infection. MDMs were left uninfected (UI) or DENV-infected, and at 48 hpi, cells were formalin fixed and then stained with (A) mouse-anti-NS1 (green) and rabbit-anti-Vav (total) (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively; (B) mouse-anti-NS1 (green) and rabbit-anti-Vav2 (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively. Nuclei were stained using Hoechst 33342 (blue). Images were captured (Olympus, IX83), and representative images are shown. (C) Images were assessed for protein colocalization by confocal microscopy (Zeiss LSM 880 with Airyscan), 63× objective lens at 2× zoom and Z-stack analysis. Two serial 0.25 µM images are shown for NS1/Vav2 staining from two different images for DENV-infected cells.

Journal: Microbiology Spectrum

Article Title: Vav proteins do not influence dengue virus replication but are associated with induction of phospho-ERK, IL-6, and viperin mRNA following DENV infection in vitro

doi: 10.1128/spectrum.02391-23

Figure Lengend Snippet: Vav1 and 2 are expressed in MDMs and not affected by DENV infection. MDMs were left uninfected (UI) or DENV-infected, and at 48 hpi, cells were formalin fixed and then stained with (A) mouse-anti-NS1 (green) and rabbit-anti-Vav (total) (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively; (B) mouse-anti-NS1 (green) and rabbit-anti-Vav2 (red) with detection of complexes using anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 555, respectively. Nuclei were stained using Hoechst 33342 (blue). Images were captured (Olympus, IX83), and representative images are shown. (C) Images were assessed for protein colocalization by confocal microscopy (Zeiss LSM 880 with Airyscan), 63× objective lens at 2× zoom and Z-stack analysis. Two serial 0.25 µM images are shown for NS1/Vav2 staining from two different images for DENV-infected cells.

Article Snippet: Images were captured by epifluorescence microscopy using an Olympus IX83 inverted microscope system equipped with a UPlanSApo 60× (1.35 N.A.) oil immersion objective, pE-800 LED light source (CoolLED), appropriate fluorescent filter sets (BrightLine Pinkel DAPI/FITC/TRITC/Cy5 and CFP/YFP/mCherry multiband filter sets; Semrock) and an iXon Life 888 EMCCD camera (Andor), controlled using cellSens Dimensions (vers.

Techniques: Infection, Staining, Confocal Microscopy

Vav2 is expressed in ARPE-19 cells and not affected by DENV-infection. ARPE-19 cells were left uninfected (UI) or DENV-infected and at 48 hpi. (A) Total RNA was extracted and subjected to qRT-PCR for Vav2 mRNA. Results were normalized to cyclophilin and then expressed relative to uninfected cells. Data represent mean ± SEM from n = 6 measures from three independent experiments. No significant difference was observed (unpaired t test with Welch’s correction). (B) Cells were fixed and immunostained for mouse-anti-DENV NS1 (green) and rabbit-anti-Vav2 (red) with detection of complexes using anti-mouse 488 and anti-rabbit 555, respectively. Nuclei were stained using Hoechst 33342 (blue). Images were captured (Olympus, IX83), and representative images are shown. (C) Images were assessed for protein colocalization by confocal microscopy (Zeiss LSM 880 with Airyscan), 63X objective lens at 2X zoom and Z-stack analysis. Two different images are shown for NS1/Vav2 staining in DENV-infected cells.

Journal: Microbiology Spectrum

Article Title: Vav proteins do not influence dengue virus replication but are associated with induction of phospho-ERK, IL-6, and viperin mRNA following DENV infection in vitro

doi: 10.1128/spectrum.02391-23

Figure Lengend Snippet: Vav2 is expressed in ARPE-19 cells and not affected by DENV-infection. ARPE-19 cells were left uninfected (UI) or DENV-infected and at 48 hpi. (A) Total RNA was extracted and subjected to qRT-PCR for Vav2 mRNA. Results were normalized to cyclophilin and then expressed relative to uninfected cells. Data represent mean ± SEM from n = 6 measures from three independent experiments. No significant difference was observed (unpaired t test with Welch’s correction). (B) Cells were fixed and immunostained for mouse-anti-DENV NS1 (green) and rabbit-anti-Vav2 (red) with detection of complexes using anti-mouse 488 and anti-rabbit 555, respectively. Nuclei were stained using Hoechst 33342 (blue). Images were captured (Olympus, IX83), and representative images are shown. (C) Images were assessed for protein colocalization by confocal microscopy (Zeiss LSM 880 with Airyscan), 63X objective lens at 2X zoom and Z-stack analysis. Two different images are shown for NS1/Vav2 staining in DENV-infected cells.

Article Snippet: Images were captured by epifluorescence microscopy using an Olympus IX83 inverted microscope system equipped with a UPlanSApo 60× (1.35 N.A.) oil immersion objective, pE-800 LED light source (CoolLED), appropriate fluorescent filter sets (BrightLine Pinkel DAPI/FITC/TRITC/Cy5 and CFP/YFP/mCherry multiband filter sets; Semrock) and an iXon Life 888 EMCCD camera (Andor), controlled using cellSens Dimensions (vers.

Techniques: Infection, Quantitative RT-PCR, Staining, Confocal Microscopy